Тest system for detecting of RNA of coronavirus strain SARS-CoV-2 using reverse transcription PCR (RT-PCR)
The set is aimed for detecting of RNA of coronavirus of strain SARS-CoV-2 in biotic substrates and based on the principle of RT-PCR. The set is developed to detect genome SARS-CoV-2 paragraphs. The positive detection control consists of the synthetic RNA SARS-CoV-2 and should be amplified together with the analyzed samples.
Collection of samples
For detecting of RNA of coronavirus strain SARS-CoV-2 is possible to withdraw the following biological substrates:
- Rhinopharynx mucosa swabs and/or oropharynx posterior wall swabs (in the presence of symptoms of the anatomical airway damage);
- expectoration or bronchoalveolar lavage (in case of tracheal intubation);
- blood plasma;
- fecal matter (in the presence of GI tract damage symptoms).
Brief review of experimental record.
- To perform a general RNA extraction using a recommended extraction set
- To prepare and to pour out in the test tubes the working solution of reagent mixture, to add the samples
- To close the test-tubes, to put them into thermo cycler, to adjust and to set into action RТ-PCR
- Growth curves analysis in samples tested, control samples and identifying standards
Total time, necessary for finishing of RТ-PCR in accordance with this protocol is approximately 1 hour 30 minutes.
